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Then a biotinylated detection antibody specific for CXCL2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
The plate has been pre-coated with Human FCN2 antibody
Substrate solution is then added and color develops in proportion to the amount of Human GPER1
And then biotinylated Human AFAMIN Antibody is added and binds to AFAMIN in the sample
Human PGAM1 (Phosphoglycerate Mutase 1) ELISA Kit Packaging:96 wells Then a biotinylated detection antibodyThe Human (PGAM1) Phosphoglycerate Mutase 1 ELISA Kit measures Phosphoglycerate mutase 1 in samples. The plate has been pre coated with Human PGAM1 antibody. PGAM1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human PGAM1 Antibody is added and binds to PGAM1 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated PGAM1 antibody. After incubation unbound Streptavidin HRP is washed
