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Then a biotinylated detection antibody specific for Human NRP1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Then Streptavidin-HRP is added and binds to the Biotinylated HYAL3 antibody
SLC39A3 present in the sample is added and binds to antibodies coated on the wells
only those wells that contain Human SIGLEC1
Human pro-BDNF (pro Brain Derived Neurotrophic Factor) ELISA Kit P8 Then a biotinylated detection antibodyThe Human pro BDNF (pro Brain Derived Neurotrophic Factor) ELISA Kit measures pro BDNF in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti pro BDNF antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components.
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