Porcine C3A (Complement Component 3a) ELISA Kit NEF Substrate solution is then added

Substrate solution is then added and color develops in proportion to the amount of Human RPS6KB1

Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to TGFb1

The concentration of Mouse TGFb1 in the samples is then determined by comparing the OD of the samples to the standard curve

Calculate the concentration of VGF in the sample by plotting standard curve

Porcine C3A (Complement Component 3a) ELISA Kit NEF Substrate solution is then addedThe Porcine C3A (Complement Component 3a) ELISA Kit measures C3a in Porcine Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti C3a antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated