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Mouse β-EP in the sample or standard competes with a fixed amount of Mouse β-EP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Mouse β-EP
The concentration of Human PCT in the samples is then determined by comparing the OD of the samples to the standard curve
IL25 present in the sample is added and binds to antibodies coated on the wells
The concentration of Mouse FLG in the samples is then determined by comparing the OD of the samples to the standard curve
Sheep PROG (Progesterone) ELISA Kit HIF1A Mouse β-EP in the sampleThe Sheep (PROG) Progesterone ELISA Kit measures Progesterone in Sheep samples. Add samples to the pre coated plate. Then add biotinylated antigen. The antigens in the samples compete with the biotinylated antigen to bind to the capture antibody and incubate. Unbound antigen is washed away during a washing step. An avidin HRP is then added and then incubate. Unbound avidin HRP is washed away during a washing step. TMB Substrate is then added and color
