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The concentration of TNF-α in the sample was calculated by drawing a standard curve
it binds with C3d bound to precoated antibody
then added a HRP-conjugated antibody specific to Human PSA
This Mouse CXCL5 (Chemokine C-X-C-Motif Ligand 5) ELISA Kit uses the sandwich-ELISA principle
Mouse RBP4 (Retinol Binding Protein 4, Plasma) ELISA Kit Packaging:96 wells The concentration of TNF-α inThe Mouse RBP4 (Retinol Binding Protein 4, Plasma) ELISA Kit measures RBP4 in Mouse Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti RBP4 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated
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