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Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Sheep IL8
Then a biotinylated detection antibody specific for Human ANXA1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
And then biotinylated Human SOX1 Antibody is added and binds to SOX1 in the sample
Substrate solution is then added and color develops in proportion to the amount of Human ENG
Chicken IGY (Immunoglobulin Y) ELISA Kit Packaging:96 wells Standards or samples are addedThe Chicken IGY (Immunoglobulin Y) ELISA Kit measures IgY in Chicken Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti IgY antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection
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