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The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse BTD
The plate has been pre-coated with Mouse GLRX5 antibody
Then a biotinylated detection antibody specific for Mouse UCP-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Substrate solution is then added and color develops in proportion to the amount of Human ART1
Rat HYP (Hydroxyproline) ELISA Kit Packaging:96 wells The microtiter plate provided inThe Rat (HYP) Hydroxyproline ELISA Kit measures hydroxyproline in Rat samples. Add samples to the pre coated plate. Then add biotinylated antigen. The antigens in the samples compete with the biotinylated antigen to bind to the capture antibody and incubate. Unbound antigen is washed away during a washing step. An avidin HRP is then added and then incubate. Unbound avidin HRP is washed away during a washing step. TMB Substrate is then added and color
