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Only those wells that contain Mouse IL-1Beta
The concentration of Human TNF-alpha in the samples is then determined by comparing the OD of the samples to the standard curve
Then a biotinylated detection antibody specific for Human MMP-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Mouse IgG are added to the micro ELISA plate wells
Collagen IV (5C9) Mouse mAb OCIF Only those wells that containCollagens are fibrous, extracellular matrix proteins with high tensile strength and are the major components of connective tissue, such as tendons and cartilage. All collagens contain a triple helix domain and frequently show lateral self association in order to form complex connective tissues. Several collagens also play a role in cell adhesion, important for maintaining normal tissue architecture and function.
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