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The concentration of Rat APOA5 in the samples is then determined by comparing the OD of the samples to the standard curve
Then a biotinylated detection antibody specific for Human CDNF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
CLTB present in the sample is added and binds to antibodies coated on the wells
Cyclin B1 Mouse Monoclonal Antibody (5C1)
Mouse HSP60 (Heat Shock Protein 60) ELISA Kit SCYA1 The concentration of Rat APOA5The Mouse (HSP60) Heat Shock Protein 60 ELISA Kit measures Heat Shock Protein 60 in Mouse samples. The plate has been pre coated with Mouse HSPD1 antibody. HSPD1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse HSPD1 Antibody is added and binds to HSPD1 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated HSPD1 antibody. After incubation unbound Streptavidin HRP is washed
