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coli expression system and the target gene encoding Gly57-Leu233 is expressed with a 6His tag at the N-terminus
then added a HRP-conjugated antibody specific to Rat 25-OH-D to each microplate well and incubated
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Ab1-42
The concentration of Human IL-8 in the samples is then determined by comparing the OD of the samples to the standard curve
Mini-sample Mouse IL-12 p40 (Interleukin 12 p40) ELISA Kit Nucleic Acid Purification coli expression system and theThis Interleukin 12 p40 ELISA kit uses the Sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Mouse IL 12 p40. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IL 12 p40 and Avidin Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and
