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Then a biotinylated detection antibody specific for Human TIMP-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
The OD value is proportional to the concentration of Human PDGF-BB
Only those wells that contain Mouse FcεRII/CD23
The concentration of Human IL1RL1 in the samples is then determined by comparing the OD of the samples to the standard curve
IL5Ra (Interleukin 5 Receptor Alpha) ELISA Kit (Small Sample) Packaging:96 wells Then a biotinylated detection antibodyThe IL5Ra (Interleukin 5 Receptor Alpha) ELISA Kit is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre coated with an antibody specific to Human IL5Ra. Standards or samples are added to the appropriate microtiter plate wells then with a biotin conjugated antibody specific to Human IL5Ra. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate
