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CDH9 present in the sample is added and binds to antibodies coated on the wells
And then biotinylated Human CLDN12 Antibody is added and binds to CLDN12 in the sample
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Goat IL-12p40
The concentration of Human GRAP2 in the samples is then determined by comparing the OD of the samples to the standard curve
Mouse OPN (Osteopontin) ELISA Kit NPN1 CDH9 present in the sampleThe Mouse OPN (Osteopontin) ELISA Kit measures OPN in Mouse Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti OPN antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody.
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