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Only those wells that contain Human SYNPO
Then a biotinylated detection antibody specific for Mouse OPG and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat IL-1Beta
Then Streptavidin-HRP is added and binds to the Biotinylated ADAMTSL5 antibody
Rat LH (Luteinizing Hormone) ELISA Kit Packaging:96 wells Only those wells that containThe Rat (LH) Luteinizing Hormone ELISA Kit measures Luteinizing Hormone in Rat samples. Add samples to the pre coated plate. Then add biotinylated antigen. The antigens in the samples compete with the biotinylated antigen to bind to the capture antibody and incubate. Unbound antigen is washed away during a washing step. An avidin HRP is then added and then incubate. Unbound avidin HRP is washed away during a washing step. TMB Substrate is then added
