Leptin ELISA Kit UP-1 Then a biotinylated detection antibody

Then a biotinylated detection antibody specific for Mouse IP-10/CXCL10 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

You can calculate the concentration of Mouse F9 in the samples by comparing the OD of the samples to the standard curve

The concentration of Chicken IL5 in the samples is then determined by comparing the OD of the samples to the standard curve

Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit PF4

Leptin ELISA Kit UP-1 Then a biotinylated detection antibodyThe Leptin ELISA kit is a two step capture or sandwich type immunoassay to measure Leptin in serum. The assay makes use of two highly specific monoclonal antibodies: A monoclonal antibody specific for leptin is immobilized onto the microplate and another monoclonal antibody specific for a different epitope of leptin is conjugated to biotin (biotin conjugate). In the first incubation step, leptin present in the specimen samples, calibrators and